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# Alignment data for "Host translesion polymerases are required for viral genome integrity"

Preferred citation (DataCite format):

  Zeltzer, Sebastian; Longmire, Pierce Alyn; Svoboda, Marek; Bosco, Giovanni; Goodrum, Felicia (2022).
  Alignment data for "Host translesion polymerases are required for viral genome integrity".
  University of Arizona Research Data Repository.
  Dataset. https://doi.org/10.25422/azu.data.19127993


Corresponding Author:
  Felicia Goodrum, Immunobiology, fgoodrum@arizona.edu


License:
  CC0


DOI:
  https://doi.org/10.25422/azu.data.19127993



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## Summary

Human cells encode up to 14 DNA polymerases with specialized functions in
chromosomal DNA synthesis and damage repair. By contrast, complex DNA viruses,
such as those of the herpesviridae family, encode a single high-fidelity DNA
polymerase. This disparity raises the possibility that DNA viruses may rely on
host polymerases for synthesis through complex DNA geometries. We tested the
importance of error-prone Y-family polymerases involved in translesion
synthesis (TLS) to human cytomegalovirus (HCMV) infection. We find most
Y-family polymerases involved in the nucleotide insertion and bypass of
lesions restrict HCMV genome synthesis and replication. By contrast, other TLS
polymerases, such as the polymerase z complex, which extends past lesions, was
required for optimal genome synthesis and replication. Depletion of either the
polz complex or the suite of insertion polymerases demonstrate that TLS
polymerases suppress the frequency of viral genome rearrangements,
particularly at GC-rich sites and repeat sequences. Moreover, while distinct
from HCMV, replication of the related herpes simplex virus type 1 (HSV-1) is
impacted by host TLS polymerases, suggesting a broader requirement for host
polymerases for DNA virus replication. These findings reveal an unexpected
role for host DNA polymerases in ensuring viral genome stability.

These data represent alignments used for Fig. 5 of the associated publication
(https://doi.org/10.1073/pnas.2203203119). Also included is a TB40E reference genome.
Refer to the README.rtf file in Alignments.zip for further information.