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# How Cholesterol Stiffens Unsaturated Lipid Membranes


Preferred citation (DataCite format):  

 Chakraborty, Saptarshi; Doktorova, Milka; Molugu, Trivikram R.; Heberle, Frederick A.; Scott, Haden L.; Dzikovski, Boris; et al. (2020): 
 How Cholesterol Stiffens Unsaturated Lipid Membranes. 
 University of Arizona Research Data Repository. Dataset. 
 https://doi.org/10.25422/azu.data.12712856


Corresponding Author:   
  Rana Ashkar,  Department of Physics, Virginia Tech, Blacksburg, VA, USA; and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, VA, USA. ashkar@vt.edu  
  Michael F. Brown, Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA; and Department of Physics, University of Arizona, Tucson, AZ, USA, mfbrown@u.arizona.edu


License:
  CC0


DOI:
  https://doi.org/10.25422/azu.data.12712856



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## Summary

Solid-state deuterium nuclear magnetic resonance (2H NMR) spectroscopy 
data are reported. Cholesterol locally increases the bending rigidity of 
DOPC membranes, similar to saturated membranes, by increasing the 
bilayer’s packing density. Solid-state NMR is inherently sensitive to 
mesoscale bending fluctuations, showing up to a 3-fold increase in 
effective bending rigidity with increasing cholesterol content 
approaching a 50% mole fraction. Our observations are in good agreement 
with the known effects of cholesterol on the area compressibility 
modulus and membrane structure, reaffirming membrane structure-property 
relationships. 

All the raw data and results are submitted as ASCII or pdf files.



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## Files and Folders

#### NMR: Processed (Fourier transformed) NMR data files and results
- NMR_Bending_rigidity_DOPC_Chol_25c 
- NMR_Bilayer structure_DOPC_Chol_25C.pdf
- NMR_Order_parameters_25c
- NMR_QE_dopc10dpopc10ch25c
- NMR_QE_dopc10dpopc20ch25c
- NMR_QE_dopc10dpopc25c
- NMR_QE_dopc10dpopc30ch25c
- NMR_QE_dopc10dpopc40ch25c
- NMR_QE_dopc10dpopc50ch25c
- NMR_QE_dpopc25c
- NMR_QE_popc10dpopc25c
- NMR_QE_popc10dpopc50ch25c
- NMR_Squarelaw_DOPC_Chol25c
- NMR_Structure_DOPC_Chol_25c
- NMR_T1z_dopc10dpopc10ch25c
- NMR_T1z_dopc10dpopc20ch25c
- NMR_T1z_dopc10dpopc25c
- NMR_T1z_dopc10dpopc30ch25c
- NMR_T1z_dopc10dpopc40ch25c
- NMR_T1z_dopc10popc50ch25c
- NMR_T1z_dpopc25c
- NMR_T1z_popc10dpopc25c
- NMR_T1z_popc10dpopc50ch25c