C. elegans bacteria colonization data set. Wild type (N2) vs. pmk-1 knockout (KU25) worms colonized with GFP-labeled E. coli OP50 bacteria

This is a C. elegans dataset used to test and validate the Systematic Imaging of Caenorhabditis Killing Organisms (SICKO) imaging and analysis pipeline. The dataset consists of raw TIF image files containing fluorescent images of individual C. elegans cultured in custom single-worm culture environments (DOI: 10.3791/64682). Wild type (strain N2) or pmk-1 knockout (strain KU25) C. elegans were challenged with GFP-labeled E. coli OP50 bacteria, washed to remove non-adherent bacteria, and transferred to single-worm culture environment wells with unlabeled bacteria. Each worm was imaged daily to quantify fluorescent intestinal bacterial colonies.