University of Arizona
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Aedes aegypti mosquito eggshell LC-MS/MS proteomics data

posted on 2023-10-16, 13:29 authored by Jun IsoeJun Isoe

Two independent biological replicates from both RNAi-Fluc and RNAi-EOF1 were used in the Aedes aegypti mosquito eggshell proteomic analysis. Ovary tissues were dissected from RNAi-mosquitoes 4days PBM. Mature primary follicles were removed from dissected ovaries and thoroughly washed in 1X PBS to completely remove shed follicular epithelial cells, secondary follicles, the germarium, and the ovarian tissues including common and lateral oviducts.The enriched eggshells were homogenized in 6.0 M guanidine hydrochloride and incubated at 37oC overnight. Eggshell protein samples were separated about 1.0 cm into resolving gel on SDS-12% PAGE gels. LC-MS/MS analysis was performed on a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, San Jose, CA) equipped with an EASY-Spray nanoESI source at the University of Arizona Analytical and Biological Mass Spectrometry Facility.

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Proteomics studies were supported by Research, Innovation & Impact Core Facilities Pilot Program at the University of Arizona to Jun Isoe


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