Organophosphate Ester Concentration & Ancillary Data for "Organophosphate Ester Transport and Microbial Responses Along the Amazon River-North Atlantic Ocean Continuum"

This data is part of the submitted manuscript “Organophosphate Ester Transport and Microbial Responses Along the Amazon River-North Atlantic Ocean Continuum”; by Waggoner et al. This dataset includes two sampling types, both taken in the Atlantic Ocean and into the Amazon River Plume. This data was collected by EM. Waggoner onboard the research vessel F/S Meteor (http://doi.org/10.48433/cr_m174) between April 12th and May 30th of 2021.

1. Dataset one (Excel file ‘Waggoner_2025_Transects.xlsx’) includes samples from 18 stations spanning three transects. At each station, several parameters were collected. Organophosphate ester concentrations were determined: surface seawater (2.4 liters) was collected using an inox collector and transferred into glass bottles. Seawater was filtered and passed through a solid phase extraction column. Four OPEs were quantified: Tris (2-chloroethyl) phosphate [TCEP], triphenyl phosphate [TPhP], tris (2-chloroisopropyl) phosphate [TCPP], and tri-isobutyl phosphate [TiBP]. Alkaline phosphatase activity [APA], soluble reactive phosphorus [SRP], particulate phosphorus [PartP], and dissolved organic phosphorus [DOP] were measured in parallel. Additionally, seawater was collected for Synechococcus [Syn], Prochlorococcus [Pro], picoeukaryotes [Pico Euks], and bacteria [Het bac] cell enumeration. All samples were frozen and transported to the University of Arizona for analysis (see manuscript methods section for more detail). The station name [Station], date, latitude [LAT], longitude [LONG], and salinity are included as contextual parameters.

2. Dataset two (Excel file ‘Waggoner_2025_AmendmentExperiments.xlsx’) includes OPE amendment experimental data. To investigate picoplankton group responses to individual OPE exposure, OPE amendment experiments were carried out at five stations, representing two open-ocean stations, two stations along the ARP, and one station in the mouth of the Amazon River (Table S1). Each experiment consisted of duplicate seawater incubation bottles with amendments of either TCEP, TPhP, or Pi (as KH₂PO₄) at 100 ng L⁻¹ final concentration. An unamended control was included in parallel. Both the Pi and unamended treatments were prepared with an acetone addition to match the solvent conditions of the OPE treatments. Surface seawater (2.4-L) was collected using an inox collector, transferred into glass bottles, and incubated for 72-hours in on-deck incubators. In-situ water temperature and light conditions were maintained throughout the incubation period. Samples to quantify OPEs, PartP, SRP, DOP, APA, and picoplankton abundances were collected from the incubation bottles at two time points (0 and 72-hours).

The units and parameter descriptions are listed in the second tab of each Excel file.