Exoproteomics data for "Dissolved Organic Phosphorus Bond-Class Utilization by Synechococcus"

This data is part of the submitted manuscript “Dissolved Organic Phosphorus Bond-Class Utilization by Synechococcus” by Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, and Solange Duhamel. This dataset aims to identify the enzymes expressed when picophytoplankton Synechococcus is phosphorus stressed. Synechococcus strains WH8102 (open-ocean strain isolated from the Atlantic Ocean) and WH5701 (coastal strain isolated from Maine, US) were grown in the Duhamel Lab at the University of Arizona. Axenic culture was grown in triplicate 500 mL culture flasks in SN media amended with 1 µmol L^-1 KH₂PO₄; a concentration previously determined as being low enough to induce phosphorus stress while still maintaining biomass (Cox and Saito, 2013). Peptide samples were analyzed at the Proteomics and Mass Spectrometry (PAMS) facility at the University of Georgia on a Thermo-Fisher LTQ Orbitrap Elite mass spectrometer coupled with a Proxeon Easy NanoLC system (Waltham, MA, United States) following Adams et al. (2022). Peptides were mapped to the Synechococcus genome (NCBI BioProject PRJNA230) (Palenik et al. 2003; McCarren et al. 2005). The protein set was searched in BLASTP against NCBI non-redundant databases, and accession numbers were cross-referenced on UniProt to confirm putative function and identify alkaline phosphatases.

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